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Proceedings Paper

Ultracompact high-sensitivity label-free biosensor using VCSEL
Author(s): Carlos F. R. Mateus; Michael C. Y. Huang; Jonathan Foley; P. Robert Beatty; Peter Li; Brian T. Cunningham; Connie J. Chang-Hasnain
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Paper Abstract

We report a label-free, highly sensitive biosensor using a vertical cavity surface emitting laser (VCSEL) based measurement system for the detection and monitoring of biomolecular interactions. The sensor system consists of a VCSEL, a plastic guided mode resonant (GMR) filter, and two pin detectors. The system has several advantages such as extreme compactness, high sensitivity, high throughput, fast measurements, low power consumption, low cost, and the potential to become portable. Experimentally, the biosensor system has shown to be highly sensitive to the surface modifications due to molecular bindings, with the ability to detect the thickness variations <10Å and refractive index variations <0.005. The biosensor also has demonstrated its high sensitivity for the detection of antibody-antigen proteins bindings, with the mouse IgG concentration as low as 1pg/ml (6.7 femto-Molar), and its ability for measuring both static and dynamic interactions among proteins.

Paper Details

Date Published: 22 June 2004
PDF: 7 pages
Proc. SPIE 5328, Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II, (22 June 2004); doi: 10.1117/12.531788
Show Author Affiliations
Carlos F. R. Mateus, Univ. of California/Berkeley (United States)
Michael C. Y. Huang, Univ. of California/Berkeley (United States)
Jonathan Foley, Univ. of California/Berkeley (United States)
P. Robert Beatty, Univ. of California/Berkeley (United States)
Peter Li, SRU Biosystems (United States)
Brian T. Cunningham, SRU Biosystems (United States)
Connie J. Chang-Hasnain, Univ. of California/Berkeley (United States)

Published in SPIE Proceedings Vol. 5328:
Microarrays and Combinatorial Techniques: Design, Fabrication, and Analysis II
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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