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Proceedings Paper

Pulsed photodynamic inactivation of gram-negative bacteria: in vitro study using methylene blue
Author(s): Masafumi Nagasawa; Shunichi Sato; Satoko Kawauchi; Nariyoshi Shinomiya; Daizo Saito; Hiroshi Ashida; Minoru Obara
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Paper Abstract

Pulsed photodynamic effect on Ps.aeruginosa has been investigated in vitro with the use of methylene blue (MB) as a photosensitizer. Bacterial suspensions were illuminated with 665-nm, nanosecond light pulses under the various drug and light dose conditions. Bacterial survival fraction decreased with increasing the drug and light doses, while no significant bacterial cell death was observed when the bacteria were washed after incubation with the drug. These results suggest a two-step photo-bactericidal mechanism; the photodynamic effect first induces membrane damage through which MB molecules were translocated into the bacterial cytoplasm, and then caused bacterial cell death.

Paper Details

Date Published: 14 June 2004
PDF: 4 pages
Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); doi: 10.1117/12.530817
Show Author Affiliations
Masafumi Nagasawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Satoko Kawauchi, National Defense Medical College (Japan)
Nariyoshi Shinomiya, National Defense Medical College (Japan)
Daizo Saito, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)


Published in SPIE Proceedings Vol. 5315:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII
David Kessel, Editor(s)

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