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Proceedings Paper

In vitro gene transfer by the application of laser-induced stress wave: effect of cellular heating
Author(s): Mitsuhiro Terakawa; Shunichi Sato; Makoto Ogura; Kuniaki Nakanishi; Yoshinori Masaki; Maki Uenoyama; Hitoshi Wakisaka; Hiroshi Ashida; Minoru Obara
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Paper Abstract

We previously demonstrated in vitro that the simultaneous application of cellular heating and a laser-induced stress wave (LISW) enhanced the uptake of porfimer sodium (Photofrin) by cells. In this study, we attempted to apply this technique to gene transfer to cultured cells. LISW and/or a transient mild (~43°C) heating been applied to deliver plasmid coding for green fluorescent protein (EGFP) to NIH-3T3 cells. It was found that simultaneous application of an LISW and the heating significantly increased the transfection efficiency by a factor of 2.5 when compared with that for the cells treated with an LISW alone.

Paper Details

Date Published: 1 July 2004
PDF: 4 pages
Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); doi: 10.1117/12.530815
Show Author Affiliations
Mitsuhiro Terakawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College (Japan)
Makoto Ogura, Keio Univ. (Japan)
Kuniaki Nakanishi, National Defense Medical College (Japan)
Yoshinori Masaki, National Defense Medical College (Japan)
Maki Uenoyama, National Defense Medical College (Japan)
Hitoshi Wakisaka, National Defense Medical College (Japan)
Hiroshi Ashida, National Defense Medical College (Japan)
Minoru Obara, Keio Univ. (Japan)

Published in SPIE Proceedings Vol. 5322:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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