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Proceedings Paper

Photodynamic inactivation of somatic frog nerve ex vivo
Author(s): Garif G. Akchurin; George A. Seliverstov; George G. Akchurin; Svetlana Yu. Kudryashova
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Paper Abstract

New techniques research mechanisms of photdynamic reactions at somatic frog nerve was approved. Dosimetry PDT with minimum time resolution ~1ms determined by changing the amplitude of compound action potential of somatic frog nerve. Light-induced inactivation of dynamic response of somatic frog nerve on electrical pulsed excitation was study ex vivo. The light-sensitive dyes: methylene blue (Mb), Indocianin green and eryhtrocin-B has been used on photodynamic induced inactivation of the processes generation nerve pulses. Inactivation of consequence action potential of somatic frog nerve using excitation of electical pulsed was achieved by irradiation with He-Ne laser light in a red spectral region (λ=633 nm, power level 2-20 mW), diode laser (λ=805 nm, P<0.1-1 W/cm2) in the case of Indocianin green and YAG:Nd laser (λ=532 nm, P~1mW) for eryhtrocin-B. It was discovered that methylene blue, Indocainine green and erytrocin-B decrease of the amplitude compound action potential of the ensemble neurons. The possible cell death mechanism was connected with damage of the sodium potassium adenosine triphosphatase (K-Na ATP) active transport which decrease of amplitude of compound action potential and decrease lifetime ionic channel of membrane nerve.

Paper Details

Date Published: 14 June 2004
PDF: 5 pages
Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); doi: 10.1117/12.530743
Show Author Affiliations
Garif G. Akchurin, Saratov State Univ. (Russia)
George A. Seliverstov, Saratov State Medical Univ. (Russia)
George G. Akchurin, Saratov State Univ. (Russia)
Svetlana Yu. Kudryashova, Saratov State Univ. (Russia)


Published in SPIE Proceedings Vol. 5315:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII
David Kessel, Editor(s)

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