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Proceedings Paper

Observation of microarray DNA hybridization using surface plasmon resonance phase-shift interferometry
Author(s): Shean-Jen Chen; C.-Y. Tsou; Y.-K. Chen; Y.-T. Su
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Paper Abstract

Surface plasmon resonance phase-shift interferometry (SPR-PSI) is a novel technique which combines SPR and modified Mach-Zehnder phase-shifting interferometry to measure the spatial phase variation caused by biomolecular interactions upon a sensing chip. The SPR-PSI imaging system offers high resolution and high-throughout screening capabilities for microarray DNA hybridization without the need for additional labeling, and provides valuable real-time quantitative information. Current SPR-PSI imaging systems measure the spatial phase variation caused by tiny biomolecular changes on the sensing interface by means of a five-step phase reconstruction algorithm and a novel multichannel least mean squares (MLMS) phase unwrapping algorithm. The SPR-PSI imaging system has an enhanced detection limit of 2.5 × 10-7 refraction index change, a long-term phase stability of π/100 in 30 minutes, and a spatial phase resolution of π/500 with a lateral resolution of 10μm. This study successfully demonstrates the kinetic and label-free observation of 5-mer DNA microarray hybridization.

Paper Details

Date Published: 16 June 2004
PDF: 8 pages
Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); doi: 10.1117/12.530568
Show Author Affiliations
Shean-Jen Chen, National Cheng Kung Univ. (Taiwan)
C.-Y. Tsou, National Central Univ. (Taiwan)
Y.-K. Chen, National Central Univ. (Taiwan)
Y.-T. Su, National Central Univ. (Taiwan)

Published in SPIE Proceedings Vol. 5327:
Plasmonics in Biology and Medicine
Tuan Vo-Dinh; Zygmunt Gryczynski; Joseph R. Lakowicz, Editor(s)

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