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Proceedings Paper

N-acetylcysteine and acute retinal laser lesions in the colubrid snake eye
Author(s): William R. Elliott; Heike K. Rentmeister-Bryant; Norman Barsalou; Jeremy Beer; Harry Zwick
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Paper Abstract

This study examined the role of oxidative stress and the effect of a single dose treatment with N-Acetylcysteine (NAC) on the temporal development of acute laser-induced retinal injury. We used the snake eye/Scanning Laser Ophthalmoscope (SLO) model, an in vivo, non-invasive ocular imaging technique, which has the ability to image cellular retinal detail and allows for studying morphological changes of retinal injury over time. For this study 12 corn-snakes (Elaphe g. guttata) received 5 laser exposures per eye, followed by either a single dose of the antioxidant NAC (150mg/kg, IP in sterile saline) or placebo. Laser exposures were made with a Nd: VO4 DPSS, 532nm laser, coaxially aligned to the SLO. Shuttered pulses were 20msec x 50 mW; 1mJ each. Retinal images were taken using a Rodenstock cSLO and were digitally recorded at 1, 6, 24-hrs, and at 3-wks post-exposure. Lesions were assessed by two raters blind to the conditions of the study yielding measures of damaged area and counts of missing or damaged photoreceptors. Treated eyes showed a significant beneficial effect overall, and these results suggest that oxidative stress plays a role in laser-induced retinal injury. The use of NAC or a similar antioxidant shows promise as a therapeutic tool.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); doi: 10.1117/12.530362
Show Author Affiliations
William R. Elliott, Naval Health Research Ctr. Detachment (United States)
Heike K. Rentmeister-Bryant, Naval Health Research Ctr. Detachment (United States)
Norman Barsalou, Naval Health Research Ctr. Detachment (United States)
Jeremy Beer, Naval Health Research Ctr. Detachment (United States)
Harry Zwick, Army Medical Research Detachment (United States)

Published in SPIE Proceedings Vol. 5319:
Laser Interaction with Tissue and Cells XV
Steven L. Jacques; William P. Roach, Editor(s)

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