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Proceedings Paper

Microcavitation and spot size dependence for damage of artificially pigmented hTERT-RPE1 cells
Author(s): Brian Matthew Mills; Tracie M. Connor; Michael S. Foltz; Jacob Stolarski; Kristy L. Hayes; Michael L. Denton; Debbie M. Eikum; Gary D. Noojin; Benjamin A. Rockwell
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Paper Abstract

We performed measurements to validate damage threshold trends in minimum visible lesion (MVL) studies as a function of spot size for nanosecond laser pulses. At threshold levels, nanosecond pulses produce microcavitation bubbles that expand and collapse around individual melanosomes. This microcavitation process damages the membranes of retinal pigment epithelium (RPE) cells. A spot size study on retinal explants found cell damage fluence (energy/area) thresholds were independent of spot size when microcavitation caused the damage, contradicting past in vivo retinal spot size experiments. The explant study (ex vivo) used a top-hat beam profile, whereas the in vivo studies used Gaussian beams. The difference in spot size trends for damage in vivo versus ex vivo may be attributed to the optics of the eye but this has not been validated. In this study, we exposed artificially pigmented human RPE cells (hTERT-RPE1)-in vitro-to 7 ns pulsed irradiation from a Ti:Sa TSA-02 regenerative amplifier (1055 nm) with beam diameters of 44, 86, and 273 μm (Gaussian beam profiles). We detected the microcavitation event with strobe illumination and time-resolved imaging. We used the fluorescent indicator dye calcein-AM, with excitation by an Argon laser (488 nm), to assess cell damage. Our current results follow trends found in the in vivo studies.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); doi: 10.1117/12.530331
Show Author Affiliations
Brian Matthew Mills, Air Force Research Lab. (United States)
Tracie M. Connor, Air Force Research Lab. (United States)
Michael S. Foltz, Northrop Grumman Corp. (United States)
Jacob Stolarski, Air Force Research Lab. (United States)
Kristy L. Hayes, Air Force Research Lab. (United States)
Michael L. Denton, Northrop Grumman Corp. (United States)
Debbie M. Eikum, Northrop Grumman Corp. (United States)
Gary D. Noojin, Northrop Grumman Corp. (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)


Published in SPIE Proceedings Vol. 5319:
Laser Interaction with Tissue and Cells XV
Steven L. Jacques; William P. Roach, Editor(s)

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