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Proceedings Paper

A compact OPO/SFG laser for ultraviolet biological sensing
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Paper Abstract

A compact parametric oscillator (OPO) with intracavity sum-frequency generation (SFG) to generate 293 nm UV laser irradiation, was developed. The OPO/SFG device was pumped by a 100 Hz Nd:YAG laser (1064 nm) of own design, including subsequent second harmonic generation (SHG) in an external periodically poled KTiOPO4 (KTP) crystal. The whole system could be used to deliver more than 30 μJ laser irradiation per pulse (100 Hz) at 293 nm. The UV laser light was introduced in an optical fiber attached to a sample compartment allowing detection of fluorescence emission using a commercial spectrometer. Aqueous samples containing biomolecules (ovalbumin) or bacteria spores (Bacillus subtilis) were excited by the UV-light at 293 nm resulting in strong fluorescence emission in the range 325 - 600 nm.

Paper Details

Date Published: 8 July 2004
PDF: 9 pages
Proc. SPIE 5332, Solid State Lasers XIII: Technology and Devices, (8 July 2004); doi: 10.1117/12.530292
Show Author Affiliations
Mikael Tiihonen, Royal Institute of Technology (Sweden)
Valdas Pasiskevicius, Royal Institute of Technology (Sweden)
Fredrik Laurell, Royal Institute of Technology (Sweden)
Per Jonsson, Swedish Defense Research Agency (Sweden)
Mikael Lindgren, Norwegian Univ. of Science and Technology (Norway)


Published in SPIE Proceedings Vol. 5332:
Solid State Lasers XIII: Technology and Devices
Richard Scheps; Hanna J. Hoffman, Editor(s)

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