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Proceedings Paper

Metal-enhanced fluorescent biomarkers for cardiac risk assessment
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Paper Abstract

In this presentation we describe a novel methodology for ultra-sensitive fluorescence immunoassays based on a new class of fluorescent biomarkers, which are strongly enhanced by nano-size metallic particles. Specifically, we discuss development of the immunoassay on the surfaces coated with metallic particles for high sensitivity detection of cardiac markers. This technology will allow detection of the biomarkers in serum and blood without separation and amplification steps. We present an experimental platform that uses front-face excitation in total internal reflection mode for efficient rejection of background fluorescence.

Paper Details

Date Published: 16 June 2004
PDF: 8 pages
Proc. SPIE 5327, Plasmonics in Biology and Medicine, (16 June 2004); doi: 10.1117/12.530091
Show Author Affiliations
Evgenia Matveeva, Univ. of Maryland/Baltimore (United States)
Zygmunt Gryczynski, Univ. of Maryland/Baltimore (United States)
Joanna B. Malicka, Univ. of Maryland/Baltimore (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore (United States)


Published in SPIE Proceedings Vol. 5327:
Plasmonics in Biology and Medicine
Tuan Vo-Dinh; Zygmunt Gryczynski; Joseph R. Lakowicz, Editor(s)

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