Share Email Print
cover

Proceedings Paper

Fluorescence lifetime imaging with a multifocal two-photon microscope
Author(s): Sandrine Leveque-Fort; Marie-Pierre Fontaine-Aupart; Gerard Roger; Patrick Georges
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Two photon microscopy is a powerful tool for cells or tissues imaging. However it presents the drawback of being a laser-scanning technique leading to long acquisition time for fluorescence lifetime imaging. Thus it is commonly limited to intensity images that only give indications on location of fluorophores but hardly reports physico-chemical properties and interactions between cells components. To preserve biological samples from too long experiments and provide a more complete spectroscopic tool we developed a time-resolved multifocal multiphoton microscope. This setup allows us to speed up the acquisition while keeping the possibility to measure both intensity and lifetime images for all multifocal points.

Paper Details

Date Published: 21 June 2004
PDF: 9 pages
Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); doi: 10.1117/12.529912
Show Author Affiliations
Sandrine Leveque-Fort, Lab. de Photophysique Moleculaire/CNRS (France)
Ctr. Universitaire (France)
Marie-Pierre Fontaine-Aupart, Lab. de Photophysique Moleculaire/CNRS (France)
Ctr. Universitaire (France)
Gerard Roger, Lab. Charles Fabry de l'Institut d'optique, CNRS (France)
Univ. Paris-Sud (France)
Ctr. Universitaire (France)
Patrick Georges, Lab. Charles Fabry de l'Institut d'optique, CNRS (France)
Univ. Paris-Sud (France)
Ctr. Universitaire (France)


Published in SPIE Proceedings Vol. 5323:
Multiphoton Microscopy in the Biomedical Sciences IV
Ammasi Periasamy; Peter T. C. So, Editor(s)

© SPIE. Terms of Use
Back to Top