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Proceedings Paper

Validation of a time-resolved fluorescence spectroscopy apparatus in a rabbit atherosclerosis model
Author(s): Qiyin Fang; Javier A. Jo; Thanassis Papaioannou; Amir Dorafshar; Todd Reil; Jian-Hua Qiao; Michael C. Fishbein; Julie A. Freischlag; Laura Marcu
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Paper Abstract

Time-resolved laser-induced fluorescence spectroscopy (tr-LIFS) has been studied as a potential tool for in vivo diagnosis of atherosclerotic lesions. This study is to evaluate the potential of a compact fiber-optics based tr-LIFS instrument developed in our laboratory for in vivo analysis of atherosclerotic plaque composition. Time-resolved fluorescence spectroscopy studies were performed in vivo on fifteen New Zealand White rabbits (atherosclerotic: N=8, control: N=7). Time-resolved fluorescence spectra were acquired (range: 360-600 nm, increment: 5 nm, total acquisition time: 65 s) from normal aorta wall and lesions in the abdominal aorta. Data were analyzed in terms of fluorescence emission spectra and wavelength specific lifetimes. Following trichrome staining, tissue specimens were analyzed histopathologically in terms of intima/media thickness and biochemical composition (collagen, elastin, foam cells, and etc). Based on intimal thickness, the lesions were divided into thin and thick lesions. Each group was further separated into two categories: collagen rich lesions and foam cell rich lesions based on their biochemical composition. The obtained spectral and time domain fluorescence signatures were subsequently correlated to the histopathological findings. The results have shown that time-domain fluorescence spectral features can be used in vivo to separate atherosclerotic lesions from normal aorta wall as well discrimination within certain types of lesions.

Paper Details

Date Published: 13 July 2004
PDF: 6 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.529677
Show Author Affiliations
Qiyin Fang, Cedars-Sinai Medical Ctr. (United States)
Javier A. Jo, Cedars-Sinai Medical Ctr. (United States)
Thanassis Papaioannou, Cedars-Sinai Medical Ctr. (United States)
Amir Dorafshar, David Geffen School of Medicine, Univ. of California/Los Angeles (United States)
Todd Reil, David Geffen School of Medicine, Univ. of California/Los Angeles (United States)
Jian-Hua Qiao, David Geffen School of Medicine, Univ. of California/Los Angeles (United States)
Michael C. Fishbein, David Geffen School of Medicine, Univ. of California/Los Angeles (United States)
Julie A. Freischlag, David Geffen School of Medicine, Univ. of California/Los Angeles (United States)
Laura Marcu, Cedars-Sinai Medical Ctr. (United States)
Univ. of Southern California (United States)


Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Reza S. Malek; Abraham Katzir; Lawrence S. Bass; Karen M. McNally-Heintzelman; Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Steen J. Madsen; David S. Robinson; Keith D. Paulsen; Kenneth Eugene Bartels; Lloyd P. Tate; Eugene A. Trowers; Werner T.W. de Riese, Editor(s)

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