Share Email Print
cover

Proceedings Paper

Optical tweezers study of viscoelastic properties in the outer hair cell plasma membrane
Author(s): David R. Murdock; Sergey A. Ermilov; Feng Qian; William E. Brownell; Bahman Anvari
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

An optical tweezers system was used to study the mechanical characteristics of the outer hair cell (OHC) lateral wall by forming plasma membrane tethers. A 2nd order generalized Kelvin model was applied to describe the viscoelastic behavior of OHC membrane tethers. The measured parameters included equilibrium tethering force, (Feq), force relaxation times (τ), stiffness values (κ), and coefficients of friction (μ). An analysis of force relaxation in membrane tethers indicated that the force decay is a biphasic process containing both an elastic and a viscous phase. In general, we observed an overall negative trend in the measured parameters upon application of the cationic amphipath chlorpromazine (CPZ). CPZ was found to cause up to a 40 pN reduction in Feq in OHCs. A statistically significant reduction in relaxation times and coefficients of friction was also observed, suggesting an increase in rate of force decay and a decrease in plasma membrane viscosity.

Paper Details

Date Published: 1 June 2004
PDF: 8 pages
Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); doi: 10.1117/12.529597
Show Author Affiliations
David R. Murdock, Rice Univ. (United States)
Sergey A. Ermilov, Rice Univ. (United States)
Feng Qian, Rice Univ. (United States)
William E. Brownell, Baylor College of Medicine (United States)
Bahman Anvari, Rice Univ. (United States)


Published in SPIE Proceedings Vol. 5331:
Nanobiophotonics and Biomedical Applications
Alexander N. Cartwright, Editor(s)

© SPIE. Terms of Use
Back to Top