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Proceedings Paper

Photo-oxidation from mode-locked laser exposure to hTERT-RPE1 cells
Author(s): Michael L. Denton; Debbie M. Eikum; Gary D. Noojin; David J. Stolarski; Randolph D. Glickman; Benjamin A. Rockwell
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Paper Abstract

Human retinal pigment epithelial (RPE) cells (hTERT-RPE1) were used to detect photo-oxidation products generated from chronic NIR (810 nm) laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H2DCF-DA and its analog CM-H2DCF-DA. Fluorescence was detected in cells exposed to mode-locked (76 MHz, ~160 femtoseconds) but not CW laser exposure. Detection of photo-oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H2DCF-DA dye proved a more sensitive indicator of oxidation than H2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures (using the most sensitive fluorescent dye conditions) when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode-locked exposure.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
Proc. SPIE 5319, Laser Interaction with Tissue and Cells XV, (1 July 2004); doi: 10.1117/12.529355
Show Author Affiliations
Michael L. Denton, Northrop Grumman Corp. (United States)
Debbie M. Eikum, Northrop Grumman Corp. (United States)
Gary D. Noojin, Northrop Grumman Corp. (United States)
David J. Stolarski, Northrop Grumman Corp. (United States)
Randolph D. Glickman, Univ. of Texas Health Science Ctr./San Antonio (United States)
Benjamin A. Rockwell, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 5319:
Laser Interaction with Tissue and Cells XV
Steven L. Jacques; William P. Roach, Editor(s)

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