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Proceedings Paper

Active particle manipulation with four laser beams
Author(s): Jan Huisken; Jim Swoger; Ernst H. K. Stelzer
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Paper Abstract

Trapping and active movement of the sample in a microscope is gently performed by applying optical forces. Optical tweezers, including the recently developed dynamic multiple-beam optical tweezers, have been shown to be indispensable tools in several fields. We demonstrate an optical trap that is formed by four laser beams. In our Differential Active Optical Manipulator (DAOM) the beams are arranged along the axes of a tetrahedron and are approximately collimated within the vicinity of their point of intersection. The DAOM makes use of a distinctive advantage of collimated light: in contrast to optical tweezers, this instrument allows movement of the confined particle over long distances without mechanical scanning. The beams' scattering forces superimpose in their common volume, and the total force (magnitude and direction) on the particle can be set by adjusting the beams' intensities. This can be done quickly and independently for each beam, allowing us to move a particle along arbitrary paths within a volume of about 1nℓ. Active feedback ensures that the sample can be held in place or transported to a specified position as desired. This allows focusing and lateral translation of the microscopic sample, which are common tasks in microscopy. During manipulation the particle is simultaneously observed from four sides. This opens new perspectives for 3D microscopy combined with contactless manipulation of the sample under observation.

Paper Details

Date Published: 1 July 2004
PDF: 8 pages
Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); doi: 10.1117/12.529171
Show Author Affiliations
Jan Huisken, European Molecular Biology Lab. (Germany)
Jim Swoger, European Molecular Biology Lab. (Germany)
Ernst H. K. Stelzer, European Molecular Biology Lab. (Germany)


Published in SPIE Proceedings Vol. 5322:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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