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Proceedings Paper

A whole organ serial sectioning and imaging system for correlation of pathology to computer tomography
Author(s): Jessica de Ryk; Eman Namati; Joseph M. Reinhardt; Christopher Piker; Ye Xu; Le Liu; Eric A. Hoffman; Geoffrey McLennan
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Paper Abstract

Randomly selected pathology sections of lung tissue are used to correlate lung pathology with Computer Tomography (CT) images. The randomly selected pathology sections provide physicians with little freedom to thoroughly investigate specific areas of interest as identified via CT images. A Large Image Microscope Array (LIMA) was designed to serially section and image entire organs for direct correlation between lung pathology and CT. The LIMA consists of a novel vibratome, capable of sectioning tissue down to a thickness of 40mm at specimen dimensions of 20cm by 30cm to a total depth of 30cm. A camera and a stereomicroscope, mounted on a XYZ gantry above the vibratome is moved through an automated raster scan to capture the entire surface area of the tissue via many high magnification images. A custom software program was developed to automate all hardware components. The alignment and stitching of the images is achieved though custom C++ code in conjunction with the Insight Segmentation and Registration Toolkit (ITK). The resulting high magnification, high-resolution pathology images are registered with corresponding CT images. Through point-to-point correlation between the two imaging techniques a pathological and CT ground truth may be established.

Paper Details

Date Published: 13 July 2004
PDF: 11 pages
Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.529137
Show Author Affiliations
Jessica de Ryk, Univ. of Iowa (United States)
Eman Namati, Flinders Univ. of South Australia (Australia)
Joseph M. Reinhardt, Univ. of Iowa (United States)
Christopher Piker, Univ. of Iowa (United States)
Ye Xu, Univ. of Iowa (United States)
Le Liu, Univ. of Iowa (United States)
Eric A. Hoffman, Univ. of Iowa (United States)
Geoffrey McLennan, Univ. of Iowa (United States)

Published in SPIE Proceedings Vol. 5324:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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