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Proceedings Paper

Slit-scanning microscope with a high-NA objective lens for analysis of synaptic function
Author(s): Takashi Sakurai; Yoshihiko Wakazono; Seiji Yamamoto; Susumu Terakawa
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Paper Abstract

By employing the total internal reflection fluorescence (TIRF) microscope with an ultra high NA (1.65) objective lens, we demonstrated detailed dynamics of exocytosis in various types of secretory vesicles. However, the TIRF microscopy could be applied to observations only on the plasma membrane and its immediate vicinity. To observe the vesicles in the deeper region of cytoplasm, we modified the TIRF optics to project a slit beam thinner than 1 μm in width to the cell. The slit beam illumination spotted single secretory vesicles inside the cell better and their movement and exocytosis easier. By scanning the slit beam, a fluorescence microscopy was possible at a high signal-to-noise ratio useful for measurement and analysis of single exocytosis in neurons and endocrine cells.

Paper Details

Date Published: 1 July 2004
PDF: 6 pages
Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); doi: 10.1117/12.529115
Show Author Affiliations
Takashi Sakurai, Hamamatsu Univ. School of Medicine (Japan)
Yoshihiko Wakazono, Hamamatsu Univ. School of Medicine (Japan)
Seiji Yamamoto, Hamamatsu Univ. School of Medicine (Japan)
Susumu Terakawa, Hamamatsu Univ. School of Medicine (Japan)


Published in SPIE Proceedings Vol. 5322:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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