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Proceedings Paper

Enhancement of spectral change from change of cell nucleus size distribution in epithelium using multiclass linear discriminant
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Paper Abstract

Here, we propose a new method to enhance the sensitivity of the reflectance spectrum to the scattering feature of the superficial tissue layer. This method is based on multiple discriminant analysis (MDA) in the eigen subspace of the spectrum. Considering the application of scattering imaging, we evaluated this method by performing multispectral imaging of two-layered tissue phantoms. A color map converted from the spectral reflectance well corresponds to variations in the size of the scatter in the first layer. In order to confirm our proposed method works well under more realistic conditions, we performed the computational simulations of the light propagation in the tissue. We used the simulation model combined with the Monte Carlo and the Mie scattering. Its conditions like the slab geometry and the particle distribution of the cell nucleus were estimated by the image measuring of pathological slices. Results on simulations show the possibility of enhancing the sensitivity of the reflectance spectrum to the scattering feature of the superficial tissue layer.

Paper Details

Date Published: 13 July 2004
PDF: 9 pages
Proc. SPIE 5326, Optical Biopsy V, (13 July 2004); doi: 10.1117/12.528902
Show Author Affiliations
Kazuhiro Gono, Olympus Corp. (Japan)
Makoto Igarashi, Tokyo Institute of Technology (Japan)
Takashi Obi, Olympus Corp. (Japan)
Masahiro Yamaguchi, Tokyo Institute of Technology (Japan)
Nagaaki Ohyama, Tokyo Institute of Technology (Japan)


Published in SPIE Proceedings Vol. 5326:
Optical Biopsy V
Robert R. Alfano; Alvin Katz, Editor(s)

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