Share Email Print
cover

Proceedings Paper

Comparison of experimental models for predicting laser-tissue interaction from 3.8-micron lasers
Author(s): Piper C. M. Williams; Golda C. H. Winston; Don Q. Randolph; Thomas A. Neal; Thomas E. Eurell; Thomas E. Johnson
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

The purpose of this study was to evaluate the laser-tissue interactions of engineered human skin and in-vivo pig skin following exposure to a single 3.8 micron laser light pulse. The goal of the study was to determine if these tissues shared common histologic features following laser exposure that might prove useful in developing in-vitro and in-vivo experimental models to predict the bioeffects of human laser exposure. The minimum exposure required to produce gross morphologic changes following a four microsecond, pulsed skin exposure for both models was determined. Histology was used to compare the cellular responses of the experimental models following laser exposure. Eighteen engineered skin equivalents (in-vitro model), were exposed to 3.8 micron laser light and the tissue responses compared to equivalent exposures made on five Yorkshire pigs (in-vivo model). Representative biopsies of pig skin were taken for histologic evaluation from various body locations immediately, one hour, and 24 hours following exposure. The pattern of epithelial changes seen following in-vitro laser exposure of the engineered human skin and in-vivo exposure of pig skin indicated a common histologic response for this particular combination of laser parameters.

Paper Details

Date Published: 13 July 2004
PDF: 7 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.528543
Show Author Affiliations
Piper C. M. Williams, Uniformed Services Univ. of the Health Sciences (United States)
Golda C. H. Winston, Uniformed Services Univ. of the Health Sciences (United States)
Don Q. Randolph, Uniformed Services Univ. of the Health Sciences (United States)
Thomas A. Neal, Uniformed Services Univ. of the Health Sciences (United States)
Thomas E. Eurell, Univ. of Illinois/Urbana-Champaign (United States)
Thomas E. Johnson, Uniformed Services Univ. of the Health Sciences (United States)


Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Reza S. Malek; Abraham Katzir; Lawrence S. Bass; Karen M. McNally-Heintzelman; Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Steen J. Madsen; David S. Robinson; Keith D. Paulsen; Kenneth Eugene Bartels; Lloyd P. Tate; Eugene A. Trowers; Werner T.W. de Riese, Editor(s)

© SPIE. Terms of Use
Back to Top