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Proceedings Paper

Virus-host interaction probed by native Stokes shift fluorescence
Author(s): Alvin Katz; Alexandra Alimova; Paul Gottlieb; Rakhi Podder; Glenn Minko; Hue Wei; Robert R. Alfano
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Paper Abstract

The fluorescence spectra from Φ6 and Φ12 cystoviruses and their pseudomonad host were investigated. The predominant fluorophore in both the Φ6 and Φ12 viruses and pseudomonas syringae is tryptophan. The emission maxima of the virus emission was found to be at 330 nm, compared to an emission maxima from the bacteria, which typically varies between 337 and 349 nm, depending on growth conditions. This difference Stokes shifts between the viruses and syringae hosts are most likely due to the virus proteins being in a much less polar environment than the bacteria proteins. This difference in Stokes shift was used to monitor the infection process.

Paper Details

Date Published: 1 July 2004
PDF: 4 pages
Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); doi: 10.1117/12.528330
Show Author Affiliations
Alvin Katz, CUNY/City College (United States)
Alexandra Alimova, CUNY/City College (United States)
Paul Gottlieb, CUNY/Graduate School and University Ctr. (United States)
Rakhi Podder, CUNY/Graduate School and University Ctr. (United States)
Glenn Minko, CUNY/City College (United States)
Hue Wei, CUNY/Graduate School and University Ctr. (United States)
Robert R. Alfano, CUNY/City College (United States)


Published in SPIE Proceedings Vol. 5321:
Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
Zygmunt Gryczynski; Anita Mahadevan-Jansen; Michael G. Sowa; Tuan Vo-Dinh; Joseph R. Lakowicz; Gerwin J. Puppels, Editor(s)

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