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Proceedings Paper

Resonance-enhanced functional third harmonic optical microscopy
Author(s): Shi-Wei Chu; Szu-Yu Chen; Tsung-Han Tsai; Tzu-Ming Liu; Shr-Bin Wu; Sajal Biring; Juen-Kai Wang; Yu-Lin Wang; Kevin Chen; Bai-Lin Lin; Chi-Kuang Sun
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Paper Abstract

For the purpose of functional third harmonic optical microscopy, it is necessary to find a method to locally enhance third harmonic generation at specific cellular site. We have demonstrated that by matching the third harmonic generation frequency of a Cr:forsterite laser and the surface plasmon resonance frequency of <50-nm silver nanoparticles, localized enhancement of third harmonic intensity of more than 100-folds can be achieved both in phantom and in real biological tissues. This strongly enhanced third harmonic signal can then be applied to specific molecule imaging by attaching the nanoparticles to the target molecule with the advantages of noninvasiveness and deep penetration capability.

Paper Details

Date Published: 21 June 2004
PDF: 8 pages
Proc. SPIE 5323, Multiphoton Microscopy in the Biomedical Sciences IV, (21 June 2004); doi: 10.1117/12.528130
Show Author Affiliations
Shi-Wei Chu, National Taiwan Univ. (Taiwan)
Szu-Yu Chen, National Taiwan Univ. (Taiwan)
Tsung-Han Tsai, National Taiwan Univ. (Taiwan)
Tzu-Ming Liu, National Taiwan Univ. (Taiwan)
Shr-Bin Wu, Institute of Atomic and Molecular Sciences (Taiwan)
Sajal Biring, Institute of Atomic and Molecular Sciences (Taiwan)
Juen-Kai Wang, Institute of Atomic and Molecular Sciences (Taiwan)
Yu-Lin Wang, Institute of Atomic and Molecular Sciences (Taiwan)
Kevin Chen, Development Ctr. for Biotechnology (Taiwan)
Bai-Lin Lin, Development Ctr. for Biotechnology (Taiwan)
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)


Published in SPIE Proceedings Vol. 5323:
Multiphoton Microscopy in the Biomedical Sciences IV
Ammasi Periasamy; Peter T. C. So, Editor(s)

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