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Proceedings Paper

Sensitive-cell-based fish chromatophore biosensor
Author(s): Thomas K Plant; Frank W.R. Chaplen; Goran Jovanovic; Wojtek Kolodziej; Janine E. Trempy; Corwin Willard; James A. Liburdy; Deborah V. Pence; Brian K. Paul
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Paper Abstract

A sensitive biosensor (cytosensor) has been developed based on color changes in the toxin-sensitive colored living cells of fish. These chromatophores are highly sensitive to the presence of many known and unknown toxins produced by microbial pathogens and undergo visible color changes in a dose-dependent manner. The chromatophores are immobilized and maintained in a viable state while potential pathogens multiply and fish cell-microbe interactions are monitored. Low power LED lighting is used to illuminate the chromatophores which are magnified using standard optical lenses and imaged onto a CCD array. Reaction to toxins is detected by observing changes is the total area of color in the cells. These fish chromatophores are quite sensitive to cholera toxin, Staphococcus alpha toxin, and Bordatella pertussis toxin. Numerous other toxic chemical and biological agents besides bacterial toxins also cause readily detectable color effects in chromatophores. The ability of the chromatophore cell-based biosensor to distinguish between different bacterial pathogens was examined. Toxin producing strains of Salmonella enteritis, Vibrio parahaemolyticus, and Bacillus cereus induced movement of pigmented organelles in the chromatophore cells and this movement was measured by changes in the optical density over time. Each bacterial pathogen elicited this measurable response in a distinctive and signature fashion. These results suggest a chromatophore cell-based biosensor assay may be applicable for the detection and identification of virulence activities associated with certain air-, food-, and water-borne bacterial pathogens.

Paper Details

Date Published: 1 July 2004
PDF: 10 pages
Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); doi: 10.1117/12.528093
Show Author Affiliations
Thomas K Plant, Oregon State Univ. (United States)
Frank W.R. Chaplen, Oregon State Univ. (United States)
Goran Jovanovic, Oregon State Univ. (United States)
Wojtek Kolodziej, Oregon State Univ. (United States)
Janine E. Trempy, Oregon State Univ. (United States)
Corwin Willard, Labtronix Inc. (United States)
James A. Liburdy, Oregon State Univ. (United States)
Deborah V. Pence, Oregon State Univ. (United States)
Brian K. Paul, Oregon State Univ. (United States)

Published in SPIE Proceedings Vol. 5321:
Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
Zygmunt Gryczynski; Anita Mahadevan-Jansen; Michael G. Sowa; Tuan Vo-Dinh; Joseph R. Lakowicz; Gerwin J. Puppels, Editor(s)

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