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Proceedings Paper

Mueller polarimetric microscopy
Author(s): Blandine Laude-Boulesteix; Antonello De Martino; Gilles Le Naour; Catherine Genestie; Laurent Schwartz; Enric Garcia-Caurel; Bernard Drevillon
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Paper Abstract

We present a multispectral polarimetric imaging system well suited for complete Mueller matrix microscopy. The source is a spectrally filtered halogen light bulb, and the image is formed on a fast CCD camera The light polarization is modulated before the sample and analyzed after the sample by using nematic liquid crystal modulators.. The whole Mueller matrix image of the sample is typically measured over 5 seconds for a good signal-to-noise ratio. The instrument design, together with an original and easy-to-operate calibration procedure provides a high polarimetric accuracy over wide ranges of wavelengths and magnifications. Mueller polarimetry provides separate images of scalar and vector retardation and dichroism of the sample, together with its depolarizing power, while all these effects do contribute simultaneously to the contrasts observed in standard polarized microsopy. Polarimetric images of several samples, namely an unstained rabbit cornea, a picrosirius red stained hepatic biopsy, and a rat artery specifically stained for collagen III are shown and discussed

Paper Details

Date Published: 13 July 2004
PDF: 12 pages
Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.527975
Show Author Affiliations
Blandine Laude-Boulesteix, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Ecole Polytechnique (France)
Antonello De Martino, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Ecole Polytechnique (France)
Gilles Le Naour, Hopital de la Pitie-Salpetriere (France)
Catherine Genestie, Hopital de la Pitie-Salpetriere (France)
Laurent Schwartz, Hopital de la Pitie-Salpetriere (France)
Enric Garcia-Caurel, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Ecole Polytechnique (France)
Bernard Drevillon, Lab. de Physique des Interfaces et des Couches Minces, CNRS (France)
Ecole Polytechnique (France)


Published in SPIE Proceedings Vol. 5324:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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