Share Email Print
cover

Proceedings Paper

Noninvasive measurement of oxygen saturation in optic-nerve head tissue
Author(s): Bahram Khoobehi; James Beach; Hiroyuki Kawano; Mark Lanoue; Deepak Chander
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We describe a non-invasive in vivo hyperspectral imaging technique for visualizing the spatial distribution of retina and optic nerve head (ONH) tissue oxygenation. Real time images of the fundus are acquired with continuous wavelengths (410-918 nm) to generate a data cube consisting of one spectral and two spatial dimensions. Reflected light from the one-dimensional (1-D) area of the sample is first passed through a grating and is then imaged onto a 12-bit silicone charge- coupled device (CCD) detector. A scanner then proceeds to the next 1-D area of the sample. Acquired image frames contain 256 spatial pixels and 256 wavelengths along rows and columns. Image sequences are scanned along the perpendicular spatial dimension using the push-broom method, whereby the spectrograph and camera are translated under constant velocity with respect to the fundus camera image over 6.6 mm of travel. This set of acquired images contains the full reflected light spectrum at each pixel of a two dimensional area of the retina and ONH. The system employs a focal plane scanner (FPS) using a linear actuator to provide motion. An algorithm processes spectral information at each pixel to represent the varying spatial distribution of retina and ONH tissue oxygenation. Imaging data are obtained from ONH tissue at both normal intraocular pressure (IOP) and acutely raised IOP.

Paper Details

Date Published: 18 June 2004
PDF: 7 pages
Proc. SPIE 5325, Optical Diagnostics and Sensing IV, (18 June 2004); doi: 10.1117/12.527803
Show Author Affiliations
Bahram Khoobehi, LSU Eye Ctr./Louisiana State Univ. Health Sciences Ctr. (United States)
James Beach, LSU Eye Ctr./Louisiana State Univ. Health Sciences Ctr. (United States)
Institute for Technology Development (United States)
Hiroyuki Kawano, LSU Eye Ctr./Louisiana State Univ. Health Sciences Ctr. (United States)
Mark Lanoue, Institute for Technology Development (United States)
Deepak Chander, LSU Eye Ctr./Louisiana State Univ. Health Sciences Ctr. (United States)


Published in SPIE Proceedings Vol. 5325:
Optical Diagnostics and Sensing IV
Gerard L. Cote; Alexander V. Priezzhev, Editor(s)

© SPIE. Terms of Use
Back to Top