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Proceedings Paper

Protoporphyrin IX distribution in rat brain following administration of 5-aminolevulinic acid or its hexylester
Author(s): Steen J. Madsen; Scott A. Friesen; Geir Olav Hjortland; Olav Engebraaten; Qian Peng; Henry Hirschberg
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Paper Abstract

The distribution of protoporphyrin IX (Pp IX) was investigated in a novel animal model followng administration of 5-aminolevulinic acid (ALA) or its hexyl ester (h-ALA). Pre-cultured tumor spheroids prepared from cell lines or tumor biopsy fragments were injected into the brains of immunodeficient rats. Approximately 3 months after spheroid implantation, Rowett nude rats were injected intra-cranially with the maximum tolerable ALA or h-ALA dose. Animals were sacrificed 4 hours post-injection and their brains removed for fluorescence microscopy analysis. The primary finding of this study is that, in the tumor biopsy model, the tumor-to-normal brain PpIX fluorescence ratio is approximately 3 times higher following direct in situ h-ALA adminstration compared to ALA.

Paper Details

Date Published: 13 July 2004
PDF: 9 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.527791
Show Author Affiliations
Steen J. Madsen, Univ. of Nevada/Las Vegas (United States)
Scott A. Friesen, Univ. of Nevada/Las Vegas (United States)
Geir Olav Hjortland, Norwegian Radium Hospital (Norway)
Olav Engebraaten, Norwegian Radium Hospital (Norway)
Qian Peng, Norwegian Radium Hospital (Norway)
Henry Hirschberg, Rikshospitalet Universitetsklinikk (Norway)

Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Reza S. Malek; Abraham Katzir; David S. Robinson; Kenneth Eugene Bartels; Eugene A. Trowers; Werner T.W. de Riese; Lawrence S. Bass; Lloyd P. Tate; Steen J. Madsen; Keith D. Paulsen; Karen M. McNally-Heintzelman, Editor(s)

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