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Proceedings Paper

Detection, imaging, and kinetics of sub-micron organelles of chondrocytes by multiple beam interference microscopy
Author(s): Narahari V. Joshi; Honorio Medina; J.M. Barboza; Gladys Colantuoni; Maritza Quintero
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Paper Abstract

Chondrocytes, obtained from testosterone treated human articular cartilage, were examined by a recently developed Multiple Beam Interference Microscopy (MBIM) attached to a confocal set up, Video-enhanced differential interference microphotography and also by cinematography. In the MBIM, the intensity of the transmitted pattern is given by the Airy function which increases the contrast dramatically as the coefficient of the reflectance of the parallel plates increases. Moreover, in this configuration, the beam passes several times through a specific organelle and increases its optical path difference both because of the increase in the trajectory and refractive index (high density) of the organelle. The improved contrast enhances the resolving power of the system and makes visible several structural details of sub micron dimensions like nucleolus, retraction fibers, podia, etc. which are not possible to reveal with such a clarity by conventional techniques such as bright field, phase contrast or DIC. This technique permits to detect the oscillatory and rotational motions of unstained cilia for the first time. The frequency of oscillations was found to be 0.8 Hz.

Paper Details

Date Published: 13 July 2004
PDF: 6 pages
Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.527544
Show Author Affiliations
Narahari V. Joshi, Hopital Universitario de Los Andes (Venezuela)
Honorio Medina, Hopital Universitario de Los Andes (United States)
J.M. Barboza, Hopital Universitario de Los Andes (Venezuela)
Gladys Colantuoni, Hopital Universitario de Los Andes (Venezuela)
Maritza Quintero, Hopital Universitario de Los Andes (Venezuela)


Published in SPIE Proceedings Vol. 5324:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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