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Proceedings Paper

Differential vascular response and relationship to tumor response with photodynamic therapy using WST-09 (TOOKAD)
Author(s): Greta M. Garbo; Peter K. Kik; Linda T. Harrison; Pierre H. Brun; Dominique Blanc; Pamela S. Paulin; Thomas Jeffery Wieman; Victor H. Fingar
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Paper Abstract

Bacteriopheophorbide molecules are second-generation photosensitizers with promise for PHotodynamic Therapy applications due largely to their absorption peaks in the near-Infrared region. Palladium bcteriopheophorbide, also called TOOKAD, has been successfully evaluated in several pre-clinical animal models. In this study the effect on tumor and normal vasculature was evaluated using an intravital vascular model on mouse cremaster muscle implanted with the RIF tumor. For tumor response studies, the same RIF tumor was implanted intradermally on the right flank and regression was evaluated for 42 days or until the tumor reached a 12 mm diameter. A light dose 300 J/cm2 were delivered at 763 nm with power density of 100 mW/cm2. Photosensitizer dose was 4 mg/kg body weight. Mice were treated immediately, 10 minutes, 30 minutes, or 24 hours after injection. Only the higher light dose (300 J/cm2) delivered 10 minutes after injection produced a reproducible and complete vascular and tumor response after PDT in these animals. In the cremaster-tumor model, arterioles and venules partially shutdown as early as 40 minutes after the beginning of treatment, while tumor neovasculature was irreversibly closed within 20 minutes of treatment. Tumor response studies demonstrated that the magnitude of vascular stasis correlates with tumor regression studies. Further studies using this photosensitizer are warranted, given its short clearance time and its near-Infrared activation wavelength.

Paper Details

Date Published: 14 June 2004
PDF: 9 pages
Proc. SPIE 5315, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII, (14 June 2004); doi: 10.1117/12.527540
Show Author Affiliations
Greta M. Garbo, Univ. of Louisville School of Medicine (United States)
Peter K. Kik, Univ. of Louisville School of Medicine (United States)
Linda T. Harrison, Univ. of Louisville School of Medicine (United States)
Pierre H. Brun, Steba Biotech (France)
Dominique Blanc, Steba Biotech (France)
Pamela S. Paulin, Univ. of Louisville School of Medicine (United States)
Thomas Jeffery Wieman, Univ. of Louisville School of Medicine (United States)
Victor H. Fingar, Univ. of Louisville School of Medicine (United States)


Published in SPIE Proceedings Vol. 5315:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XIII
David Kessel, Editor(s)

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