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Proceedings Paper

Overview on some applications of multispectral imaging autofluorescence microscopy in biology and medicine
Author(s): Giovanni Agati; Franco Fusi; Monica Monici; Riccardo Pratesi
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Paper Abstract

Many cell and tissue components exposed to light of appropriate wavelength exhibit UV-visible emission. This is a spontaneous fluorescence, defined autofluorescence in contrast with that produced by highly fluorescent exogenous probes. This communication deals with the Multispectral Imaging Autofluorescence Microscopy (MIAM) technique, that allows living cell and tissue analysis without use of any reagent or treatment modifying the native conditions of the samples. The technique is suitable for a wide range of applications, practically in all the fields where samples containing fluorescent substances occur. Examples of application in biology and medicine are given, showing the power of the technique in both basic research and diagnostics.

Paper Details

Date Published: 19 November 2003
PDF: 2 pages
Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); doi: 10.1117/12.527511
Show Author Affiliations
Giovanni Agati, Consiglio Nazionale delle Ricerche (Italy)
Franco Fusi, Univ. degli Studi di Firenze (Italy)
Monica Monici, Ctr. di Eccellenza Optronica (Italy)
Riccardo Pratesi, Univ. degli Studi di Firenze (Italy)

Published in SPIE Proceedings Vol. 4829:
19th Congress of the International Commission for Optics: Optics for the Quality of Life
Giancarlo C. Righini; Anna Consortini, Editor(s)

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