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Proceedings Paper

Repetitive 5-aminolevulinic acid mediated photodynamic therapy of rat glioma
Author(s): Henry Hirschberg; Steen J. Madsen; Even Angell-Petersen; Qian Peng; Mouldy Sioud; Chung-Ho Sun; Dag R. Sorensen
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Paper Abstract

The probability of achieving local control with current single-shot, intraoperative PDT treatments of intracerebral gliomas seems improbable due to the length of time required to deliver adequate light fluences to depths of 1 - 2 cm in the resection margin. The results of in vitro experiments indicated that PDT, repeated at weekly intervals, was substantially more effective at inhibiting glioma spheriod growth than single treatment regimes. This prompted the initiation of in vivo studies of repetitive PDT in a rat glioma model. BT4C cell line tumors were established in the brains of inbred BD-IX rats. Three days following tumor induction, the animals were injected with 250 mg/kg ALA i.p. and four hours later, after the introduction of an optical fiber, light treatment at various radiant energies was given over a 10- to 30-minute interval. Two additional treatments were given at weekly intervals. In vitro experiments verified that spheroids derived from the cell line were sensitive to ALA PDT. Microfluorometry of frozen tissue sections showed that PpIX is produced with a 10 - 20:1 tumor to normal tissue selectivity ratio four hours after 250 mg/kg ALA i.p. Toxic radiant energy levels for ALA PDT have been determined.

Paper Details

Date Published: 13 July 2004
PDF: 10 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.527395
Show Author Affiliations
Henry Hirschberg, Rikshospitalet Universitetsklinikk (Norway)
Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Steen J. Madsen, Univ. of Nevada/Las Vegas (United States)
Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Even Angell-Petersen, Norwegian Radium Hospital (Norway)
Qian Peng, Norwegian Radium Hospital (Norway)
Mouldy Sioud, Norwegian Radium Hospital (Norway)
Chung-Ho Sun, Beckman Laser Institute (United States)
Univ. of California/Irvine (United States)
Dag R. Sorensen, Rikshospitalet Universitetsklinikk (Norway)


Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Reza S. Malek; Abraham Katzir; Lawrence S. Bass; Karen M. McNally-Heintzelman; Brian Jet-Fei Wong; Nikiforos Kollias; Kenton W. Gregory; Henry Hirschberg; Steen J. Madsen; David S. Robinson; Keith D. Paulsen; Kenneth Eugene Bartels; Lloyd P. Tate; Eugene A. Trowers; Werner T.W. de Riese, Editor(s)

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