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Proceedings Paper

Progress in directed energy control of vectors for microbes and other cells
Author(s): Johnathan L. Kiel D.V.M.; Jill E. Parker; Eric A. Holwitt; Jeeva Vivekananda; Mark A. Sloan; Lucille J. V. Stribling
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Paper Abstract

Biosynthetic semiconductor, diazoluminomelanin (DALM), is a polymer of tyrosine, luminol, and nitrite. DALM has a very large cross section of absorption for light from ultraviolet to radio frequencies. This polymer can be made efficiently in a genetically engineered E.coli, JM109/pIC2ORNR1.1 (ATCC# 69905). We have been pursuing ways to couple electromagnetic radiation to vectors using this polymer. DNA capture elements (DCEs; formerly aptamers) have made this possible. We incorporated DCEs into the plasmid of this E. coli to direct binding to whatever microbe or cell desired and to produce DALM attached to the plasmid DNA. Using two other vectors pSV2neoNR101 or pSV2neoNR8005 (ATCC # 69617 and 69618, respectively), both propagated in the E. coli host HB101, we have also inserted genes necessary for DALM production into animal and human cell lines (mouse monocytic leukemia: ATCC # CRL- 11771, -11772, -1173, mouse mammary adenocarcinoma: ATCC# CRL-12184, -12185; and human carcinoma of the cervix: ATCC # CRL-12510). The DCE/DALM vectors can be used to tag target cells, detectable by broad-spectrum light absorbance, luminescence, or fluorescence. DCE/DALM can further be activated with light, microwave energy, or by oxidative chemistry to kill the targeted microbes or other cells.

Paper Details

Date Published: 13 July 2004
PDF: 8 pages
Proc. SPIE 5312, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV, (13 July 2004); doi: 10.1117/12.522922
Show Author Affiliations
Johnathan L. Kiel D.V.M., Air Force Research Lab. (United States)
Jill E. Parker, Air Force Research Lab. (United States)
Eric A. Holwitt, Air Force Research Lab. (United States)
Jeeva Vivekananda, Conceptual MindWorks, Inc. (United States)
Mark A. Sloan, Conceptual MindWorks, Inc. (United States)
Lucille J. V. Stribling, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 5312:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIV
Brian Jet-Fei Wong M.D.; Nikiforos Kollias; Kenton W. Gregory M.D.; Henry Hirschberg; Reza S. Malek M.D.; Abraham Katzir; David S. Robinson M.D.; Kenneth Eugene Bartels D.V.M.; Eugene A. Trowers M.D.; Werner T.W. de Riese; Lawrence S. Bass M.D.; Lloyd P. Tate V.D.M.; Steen J. Madsen; Keith D. Paulsen; Karen M. McNally-Heintzelman, Editor(s)

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