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Proceedings Paper

Refractive index measurement in human tissue samples
Author(s): Vesselina Tsenova; Elena V. Stoykova
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Paper Abstract

The aim of this study was to apply a laser refractometer based on a total internal reflection for measurement of refractive indices of thin tissue samples. The tissue slices with thickness from 5 to 15 μm and cross-sectional area of about 1 - 1.5 cm were cut with a freezing microtome and mounted on microscope glass slides. The samples from a liver, kidney, myocardium and skin were taken post mortem from 3 male patients (50, 52 and 75 years old) with some pathological tissue changes as well as from 3 dead fetuses 24-48 hours after the death. To indicate the morphological changes, the samples were stained with hemolown-eosin (HE) and studied microscopically. Using a two-beam UV-Vis-NIR-spectrophotometer Cary-5E (Variant), on-axis transmittance of the samples was measured over the 350 - 2000 nm range and the spectra of the extinction coefficient, in cm-1, were calculated. The measured refractive indices correspond to dehydrated tissue samples.

Paper Details

Date Published: 4 November 2003
PDF: 5 pages
Proc. SPIE 5226, 12th International School on Quantum Electronics: Laser Physics and Applications, (4 November 2003); doi: 10.1117/12.519584
Show Author Affiliations
Vesselina Tsenova, Univ. Hospital Tzaritza Ioanna (Bulgaria)
Elena V. Stoykova, Central Lab. for Optical Storage and Processing of Information (Bulgaria)

Published in SPIE Proceedings Vol. 5226:
12th International School on Quantum Electronics: Laser Physics and Applications
Peter A. Atanasov; Alexander A. Serafetinides; Ivan N. Kolev, Editor(s)

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