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Proceedings Paper

Fluorescence measurements of activity associated with a molecularly imprinted polymer imprinted to dipicolinic acid
Author(s): John Anderson; Dmitry Pestov; Robert L. Fischer; Stanley Webb; Gary C. Tepper
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Paper Abstract

Steady state and lifetime fluorescence measurements were acquired to measure the binding activity associated with molecularly imprinted polymer (MIP) microparticles imprinted to dipicolinic acid. Dipicolinic acid is a unique compound associated with the sporulation phase of spore-forming bacteria (e.g., genus Bacillus and Clostridium). Vinylic monomers were polymerized in a dimethylformamide solution containing the dipicolinic acid as a template. The resulting MIP was then pulverized and size selected into small microscale particles. Samplers were adapted incorporating the MIP particles within a dialyzer (500 MW). Tests were run on replicate samples of biologically active cultures representing both stationary phase and sporulation post fermentation products in standard media. The permeability of the membrane permitted diffusion of lighter molecular weight constituents from media effluents to enter the dialyzer chamber and contact the MIP. Extractions of the media were measured using steady state and lifetime fluorescence. Results showed dramatic steady state fluorescence changes as a function of excitation, emission and intensity and an estimated lifetime of 5.8 ns.

Paper Details

Date Published: 8 March 2004
PDF: 5 pages
Proc. SPIE 5269, Chemical and Biological Point Sensors for Homeland Defense, (8 March 2004); doi: 10.1117/12.519480
Show Author Affiliations
John Anderson, U.S. Army Corps of Engineers (United States)
Dmitry Pestov, Virginia Commonwealth Univ. (United States)
Robert L. Fischer, U.S. Army Corps of Engineers (United States)
Stanley Webb, Virginia Commonwealth Univ. (United States)
Gary C. Tepper, Virginia Commonwealth Univ. (United States)

Published in SPIE Proceedings Vol. 5269:
Chemical and Biological Point Sensors for Homeland Defense
Arthur J. Sedlacek III; Richard Colton; Tuan Vo-Dinh, Editor(s)

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