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Proceedings Paper

FTIR transmission and photoacoustic spectroscopy for the statistical identification of bacteria
Author(s): Nancy S. Foster; Nancy B. Valentine; Sandra E. Thompson; Timothy J. Johnson; James E. Amonette
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Paper Abstract

We have previously reported a combined mid-infrared spectroscopic/statistical modeling approach for the discrimination and identification, at the strain level, of both sporulated and vegetative bacteria. This paper reports on the expansion of the reference spectral library: transmissive Fourier-transform mid-infrared (trans-FTIR) spectra were obtained for three Escherichia bacterial strains (E. coli RZ1032, E. coli W3110, and E. coli HB101 ATCC 33694), and two Pseudomonas putida bacterial strains (P. putida 0301 and P. putida ATCC 39169). These were combined with the previous spectral data of five Bacillus bacterial strains (B. atrophaeus ATCC 49337, B. globigii Dugway, B. thuringiensis spp. kurstaki ATCC 35866, B. subtilis ATCC 49760, and B. subtilis 6051) to form an extended library. The previously developed four step statistical model for the identification of bacteria (using the expanded library) was subsequently used on blind samples including other bacteria as well as non-biological materials. The results from the trans-FTIR spectroscopy experiments are discussed and compared to results obtained using photoacoustic Fourier-transform infrared spectroscopy (PA-FTIR). The advantages, disadvantages, and preliminary detection limits for each technique are discussed. Both methods yield promising identification of unknown bacteria, including bacterial spores, in a matter of minutes.

Paper Details

Date Published: 8 March 2004
PDF: 11 pages
Proc. SPIE 5269, Chemical and Biological Point Sensors for Homeland Defense, (8 March 2004); doi: 10.1117/12.519199
Show Author Affiliations
Nancy S. Foster, Pacific Northwest National Lab. (United States)
Nancy B. Valentine, Pacific Northwest National Lab. (United States)
Sandra E. Thompson, Pacific Northwest National Lab. (United States)
Timothy J. Johnson, Pacific Northwest National Lab. (United States)
James E. Amonette, Pacific Northwest National Lab. (United States)

Published in SPIE Proceedings Vol. 5269:
Chemical and Biological Point Sensors for Homeland Defense
Arthur J. Sedlacek III; Richard Colton; Tuan Vo-Dinh, Editor(s)

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