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Proceedings Paper

Flow cytofluorometric monitoring of leukocyte apoptosis in experimental cholera
Author(s): Ekaterina Y. Lotsmanova; Alexander L. Kravtsov; Ludmila F. Livanova; Irina M. Kobkova; Oleg S. Kuznetsov; Tatyana N. Shchukovskaya; Nina I. Smirnova; Vladimir V. Kutyrev
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Paper Abstract

Flow cytofluorometric DNA analysis was applied to determine of the relative contents of proliferative (more then 2C DNA per cell) and apoptotic (less then 2C DNA per cell) leukocytes in blood of adult rabbits, challenged with 10,000 times the 50 % effective dose of Vibrio cholerae virulent strain by the RITARD technique. It has been shown that irreversible increase the percentage of cells carrying DNA in the degradation stage brings to disbalance between the genetically controlled cell proliferation and apoptosis that leads to animal death from the cholera infection. Such fatal changes were not observed in challenging of immunized animals that were not died. Thus received data show that the flow cytofluorometric measurements may be used for detection of transgressions in homeostasis during acute infection diseases, for outlet prognosis of the cholera infection.

Paper Details

Date Published: 13 October 2003
PDF: 5 pages
Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); doi: 10.1117/12.518875
Show Author Affiliations
Ekaterina Y. Lotsmanova, Russian Anti-Plague Research Institute Microbe (Russia)
Alexander L. Kravtsov, Russian Anti-Plague Research Institute Microbe (Russia)
Ludmila F. Livanova, Russian Anti-Plague Research Institute Microbe (Russia)
Irina M. Kobkova, Russian Anti-Plague Research Institute Microbe (Russia)
Oleg S. Kuznetsov, Russian Anti-Plague Research Institute Microbe (Russia)
Tatyana N. Shchukovskaya, Russian Anti-Plague Research Institute Microbe (Russia)
Nina I. Smirnova, Russian Anti-Plague Research Institute Microbe (Russia)
Vladimir V. Kutyrev, Russian Anti-Plague Research Institute Microbe (Russia)


Published in SPIE Proceedings Vol. 5068:
Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV
Valery V. Tuchin, Editor(s)

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