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Proceedings Paper

Spectroscopic fluorescence measurements of lamb and human heart tissue in vitro
Author(s): George Filippidis; Giannis Zacharakis; G. E. Kochiadakis; S. I. Chrysostomakis; P. E. Vardas; Costas Fotakis; Theodore G. Papazoglou
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Paper Abstract

Laser-induced fluorescence spectra were obtained during the exposure of lamb heart (n=20) tissue to Argon-ion radiation (457.9nm). Fluorescence spectra from different heart compartments (the left and right atria and ventricles, the myocardium, the epicardium, and the aorta) were recorded. Simple algebraic algorithms based on the spectral intensity variation were constructed in order to detect spectral features and characterize the different cardiac compartments. Additionally, it was investigated whether each chamber exhibited constant spectral response. After the end of each experiment the lamb hearts were stored in formalin (10%). The samples were irradiated again after forty eight (48) hours in order to investigate the spectral differences that appear due to formalin conservation. Similar fluorescence measurements were taken from a limited number of human heart tissues (n=2) ex vivo.

Paper Details

Date Published: 13 October 2003
PDF: 8 pages
Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); doi: 10.1117/12.518767
Show Author Affiliations
George Filippidis, Foundation for Research and Technology-Hellas (Greece)
Giannis Zacharakis, Foundation for Research and Technology-Hellas (Greece)
G. E. Kochiadakis, Univ. Hospital of Crete (Greece)
S. I. Chrysostomakis, Univ. Hospital of Crete (Greece)
P. E. Vardas, Univ. Hospital of Crete (Greece)
Costas Fotakis, Foundation for Research and Technology-Hellas (Greece)
Theodore G. Papazoglou, Foundation for Research and Technology-Hellas (Greece)


Published in SPIE Proceedings Vol. 5068:
Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV
Valery V. Tuchin, Editor(s)

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