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Proceedings Paper

The application of the flow cytometry for determination of the action of serotonin on the antigenic composition and amount of DNA of plague microbe cultured at 37°C
Author(s): Vladimir N. Korsukov; Tatyana N. Schukovskaya; Svetlana N. Klueva; Alexander L. Kravtsov; Tatyana A. Polunina; Youri A. Popov
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Paper Abstract

The results have been obtained by flow cytometry showed the significant raise of fluorescence intensity of Yersinia pestis EV cells binding FITC-labelled plague polyclonal immunoglobins after 24 h cultivation in Hottinger broth pH 7.2 at 37 °C in the presence of increasing concentration serotonin (5-Hydroxytryptamine). The presence of serotonin (5-HT) in nutrient broth at concentration of 10-5 M, 10-8 M could modulate DNA content in 37 °C growing population of plague microbe. The effect of this action depended of 5-HT concentration.

Paper Details

Date Published: 13 October 2003
PDF: 7 pages
Proc. SPIE 5068, Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV, (13 October 2003); doi: 10.1117/12.518632
Show Author Affiliations
Vladimir N. Korsukov, Russian Anti-Plague Research Institute Microbe (Russia)
Tatyana N. Schukovskaya, Russian Anti-Plague Research Institute Microbe (Russia)
Svetlana N. Klueva, Russian Anti-Plague Research Institute Microbe (Russia)
Alexander L. Kravtsov, Russian Anti-Plague Research Institute Microbe (Russia)
Tatyana A. Polunina, Russian Anti-Plague Research Institute Microbe (Russia)
Youri A. Popov, Russian Anti-Plague Research Institute Microbe (Russia)


Published in SPIE Proceedings Vol. 5068:
Saratov Fall Meeting 2002: Optical Technologies in Biophysics and Medicine IV

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