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Proceedings Paper

Optical thin films serving biotechnology: fluorescence enhancement of DNA chip
Author(s): Pierre Barritault; Stephane Getin; Patrick Chaton; Bernard Andre; Francoise Vinet; Brigitte Fouque
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Paper Abstract

Over the past years, DNA-chip technology has exploded. Yet scientists using such devices have to face many problems. One of them, due to the very low concentration of biological species to be detected, is the weakness of fluorescence signal collected through the reading system (microscope or scanner). To solve this problem, we proposed to use optical thin films technology. We studied the potentialities of this method step by step. The first step was to be able to understand, explain and forecast the fluorescence emitted by a DNA-chip in terms of fluorescence angular patterns. A theoretical and experimental study enabled us to master this issue even in the case of multi-layers substrates. Using this knowledge we were then able to explain, through simulations, the potentialities of this new type of substrates in terms of fluorescence enhancement. Thus we showed that a theoretical enhancement of twenty-fold (compare to a glass substrate) was achievable.

Paper Details

Date Published: 25 February 2004
PDF: 9 pages
Proc. SPIE 5250, Advances in Optical Thin Films, (25 February 2004); doi: 10.1117/12.516261
Show Author Affiliations
Pierre Barritault, CEA-DRT (France)
Stephane Getin, CEA-DRT (France)
Patrick Chaton, CEA-DRT (France)
Bernard Andre, CEA-DRT (France)
Francoise Vinet, CEA-DRT (France)
Brigitte Fouque, CEA-DSV (France)

Published in SPIE Proceedings Vol. 5250:
Advances in Optical Thin Films
Claude Amra; Norbert Kaiser; H. Angus Macleod, Editor(s)

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