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Proceedings Paper

Pulsed electrochemical detection in bioanalysis: chemical fingerprinting
Author(s): William R. LaCourse
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Paper Abstract

Pulsed Electrochemical Detection (PED) is a revolutionary approach to the simple, sensitive, and direct detection of numerous polar aliphatic compounds, especially carbohydrates. This technique exploits the electrocatalytic activity of noble metal electrode surfaces to oxidize various polar functional groups. In PED, multi-step potential-time waveforms at Au and Pt electrodes realize amperometric/coulometric detection while maintaining uniform and reproducible electrode activity. The response mechanisms in PED are dominated by the surface properties of the electrode, and, as a consequence, members of each chemical class of compounds produce virtually identical voltammetric responses. Thus, the full potential is realized when combined with high performance liquid chromatography (HPLC). This paper reviews the fundamental aspects of PED and details a novel approach to the chemical "fingerprinting" of natural products. Applications include the characterization of tobacco, peptones, and bacteria.

Paper Details

Date Published: 15 March 2004
PDF: 10 pages
Proc. SPIE 5261, Smart Medical and Biomedical Sensor Technology, (15 March 2004); doi: 10.1117/12.516162
Show Author Affiliations
William R. LaCourse, Univ. of Maryland/Baltimore County (United States)


Published in SPIE Proceedings Vol. 5261:
Smart Medical and Biomedical Sensor Technology
Brian M. Cullum, Editor(s)

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