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Proceedings Paper

Using fiber optic probe and CMOS BDJ detector for microarray spot scanning
Author(s): Patrick Pittet; Loubna Hannati; Guo Neng Lu
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Paper Abstract

We have designed a cost-effective, fiber optic bundle-based detection system for microarray fluorescence measurements. A bundle, fabricated with thin-cladding fibers of 50-μm in core diameter, is used for spot excitation and collection. The collected optical signal is detected by a CMOS BDJ (Buried Double p-n Junction) detector, which can be operated either as a photodiode or as a wavelength-sensitive device. For improving measuring rate of a microarray, we have proposed a direct spot scanning technique, which is based on a prior knowledge about the predefined microarray's mask pattern, and operates to bring the bundle successively over each spot for single-point measurement. It is implemented with a microarray registration procedure to determine the spots' positions. The detection system with implemented scanning technique has been tested using microarray samples. Its scanning operation has been verified by comparing the determined spots' coordinates to the microarray image.

Paper Details

Date Published: 18 February 2004
PDF: 8 pages
Proc. SPIE 5249, Optical Design and Engineering, (18 February 2004); doi: 10.1117/12.512503
Show Author Affiliations
Patrick Pittet, LENAC, Univ. Claude Bernard Lyon 1 (France)
Loubna Hannati, LENAC, Univ. Claude Bernard Lyon 1 (France)
Guo Neng Lu, LENAC, Univ. Claude Bernard Lyon 1 (France)

Published in SPIE Proceedings Vol. 5249:
Optical Design and Engineering
Laurent Mazuray; Philip J. Rogers; Rolf Wartmann, Editor(s)

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