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Proceedings Paper

CMOS optical detector system for capillary fluorescence measurements
Author(s): Jean-Marc Galvan; Guo-Neng Lu; Patrick Pittet; Loic Blum; Beatrice Leca-Bouvier
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Paper Abstract

We present a fluorescence detection system for capillary analysis. It is designed using a CMOS BDJ (Buried Double p-n Junction) detector which can be operated either as a photodiode or as a wavelength-sensitive device. Noise-reduction techniques such as signal pre-amplification and synchronous detection are implemented to boost the sensitivity of measurements. The system indicates fluorescence intensity for concentration determination, and average wavelength of fluorescence spectrum for molecular discrimination. The system has been tested by measuring two widely used fluorophores (FITC and Rhodamine B) in different concentrations. A 407-nm blue laser diode and a 532-nm green YAG compact laser have been respectively employed for their excitation. The illuminated volume inside the capillary is about 5 nl. The best results have been obtained with FITC, enabling as low as 10-10 M to be detectable.

Paper Details

Date Published: 19 February 2004
PDF: 8 pages
Proc. SPIE 5251, Detectors and Associated Signal Processing, (19 February 2004); doi: 10.1117/12.512407
Show Author Affiliations
Jean-Marc Galvan, Ecole Superieure Chimie Physique Electronique de Lyon (France)
Guo-Neng Lu, Univ. Claude Bernard Lyon I (France)
Patrick Pittet, Univ. Claude Bernard Lyon I (France)
Loic Blum, Univ. Claude Bernard Lyon I (France)
Beatrice Leca-Bouvier, Univ. Claude Bernard Lyon I (France)


Published in SPIE Proceedings Vol. 5251:
Detectors and Associated Signal Processing
Jean-Pierre Chatard; Peter N. J. Dennis, Editor(s)

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