Share Email Print

Proceedings Paper

Point spread function engineering in confocal scanning microscopy
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Confocal scanning microscopes are imaging systems that are mainly featured by their unique depth-discrimination capacity when imaging three-dimensional objects. Along the past few years, our research group has done several attempts to improve their axial resolution by means of the so-called point-spread-function (PSF) engineering method. That is, by designing diffractive elements that properly shape the PSF. Our PSF engineering techniques have been designed to work in the nonparaxial regime and are applied both to bright-field and single-photon fluorescence confocal microscopy, and also to more elaborated architectures like 4Pi confocal microscopy.

Paper Details

Date Published: 31 December 2003
PDF: 11 pages
Proc. SPIE 5182, Wave-Optical Systems Engineering II, (31 December 2003); doi: 10.1117/12.510209
Show Author Affiliations
Manuel Martinez-Corral, Univ. de Valencia (Spain)

Published in SPIE Proceedings Vol. 5182:
Wave-Optical Systems Engineering II
Frank Wyrowski, Editor(s)

© SPIE. Terms of Use
Back to Top