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Proceedings Paper

X-ray microscopy and imaging of Caenorhabditis elegans nematode using a laser-plasma-pulsed x-ray source
Author(s): Giulio Poletti; Franceasco Orsini; Jiri Ullschmied; Jiri Skala; Bozena Kralikova; Miroslav Pfeifer; Christelle Kadlec; Tomas Mocek; A. R. Prag; F. Cotelli; C. Lora Lamia; Dimitri Batani; A. Bernardinello; Tara Desai; A. Zullini
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Paper Abstract

An experiment on Soft X-ray Contact Microscopy (SXCM) performed on Caenorhabditis elegans nematodes is discussed. This sample has been selected since it is a well studied case used as model in many biological contexts. The experiment has been performed using the iodine PALS laser source to generate pulsed soft X-rays from laser-plasma interaction, using molybdenum and gold as targets. Typical intensities on the targets exceeded 1014 W/cm2. The SXCM imprints have been recorded on Polymethilmetacrylate (PMMA) photo resists which have been chemically developed and analyzed with an Atomic Force Microscope (AFM) operating in constant force mode. The use of error signal AFM images together with topography AFM images, did allow an easier recognition of biological patterns, and the identification of observed structures with internal organs. Several organs were identified in the SXCM images, including cuticle annuli, alae, pharynx, and three different types of cell nuclei. These are the first SXCM images of multi-cellular complex organisms.

Paper Details

Date Published: 7 January 2004
PDF: 8 pages
Proc. SPIE 5196, Laser-Generated and Other Laboratory X-Ray and EUV Sources, Optics, and Applications, (7 January 2004); doi: 10.1117/12.508377
Show Author Affiliations
Giulio Poletti, Istituto di Fisiologia Generale e Chimica Biologica (Italy)
INFM (Italy)
Unv. degli Studi di Milano (Italy)
Franceasco Orsini, Istituto di Fisiologia Generale e Chimica Biologica (Italy)
INFM (Italy)
Univ. degli Studi di Milano (Italy)
Jiri Ullschmied, PALS Research Ctr. (Czech Republic)
Jiri Skala, PALS Research Ctr. (Czech Republic)
Bozena Kralikova, PALS Research Ctr. (Czech Republic)
Miroslav Pfeifer, PALS Research Ctr. (Czech Republic)
Christelle Kadlec, PALS Research Ctr. (Czech Republic)
Tomas Mocek, PALS Research Ctr. (Czech Republic)
A. R. Prag, PALS Research Ctr. (Czech Republic)
F. Cotelli, Univ. degli Studi di Milano (Italy)
C. Lora Lamia, Univ. degli Studi di Milano (Italy)
Dimitri Batani, INFM (Italy)
Univ. degli Studi di Milano-Bicocca (Italy)
A. Bernardinello, INFM (Italy)
Univ. degli Studi di Milano-Bicocca (Italy)
Tara Desai, INFM (Italy)
Univ. degli Studi di Milano-Bicocca (Italy)
A. Zullini, Univ. degli Studi di Milano-Bicocca (Italy)


Published in SPIE Proceedings Vol. 5196:
Laser-Generated and Other Laboratory X-Ray and EUV Sources, Optics, and Applications
George A. Kyrala; Jean-Claude J. Gauthier; Carolyn A. MacDonald; Ali M. Khounsary, Editor(s)

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