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Proceedings Paper

Functional mapping of cell surface proteins with localized stimulation of single cells
Author(s): Bingyun Sun; Daniel T. Chiu
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Paper Abstract

This paper describes the development of using individual micro and nano meter-sized vesicles as delivery vessels to functionally map the distribution of cell surface proteins at the level of single cells. The formation of different sizes of vesicles from tens of nanometers to a few micrometers in diameter that contain the desired molecules is addressed. An optical trap is used to manipulate the loaded vesicle to specific cell morphology of interest, and a pulsed UV laser is used to photo-release the stimuli onto the cell membrane. Carbachol activated cellular calcium flux, upon binding to muscarinic acetylcholine receptors, is studied by this method, and the potential of using this method for the functional mapping of localized proteins on the cell surface membrane is discussed.

Paper Details

Date Published: 14 November 2003
PDF: 8 pages
Proc. SPIE 5222, Nanocrystals, and Organic and Hybrid Nanomaterials, (14 November 2003); doi: 10.1117/12.506906
Show Author Affiliations
Bingyun Sun, Univ. of Washington (United States)
Daniel T. Chiu, Univ. of Washington (United States)


Published in SPIE Proceedings Vol. 5222:
Nanocrystals, and Organic and Hybrid Nanomaterials
Alexander N. Cartwright; Charles Y. C. Lee; David L. Andrews; Zeno Gaburro, Editor(s)

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