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Proceedings Paper

Fluorescence imaging in photodynamic therapy
Author(s): Alexander J. MacRobert; David Phillips
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Paper Abstract

A quantitative technique based on fluorescence microscopic detection has been developed for measurements of photosensitizer distributions in sections of tissue and cultured cells. Imaging of photosensitizer fluorescence was achieved with negligible interference from sensitizer photodegradation and tissue autofluorescence using laser excitation and detection with a highly sensitive CCD (charge-coupled device) camera system. Techniques for the measurement of sensitizer fluorescence decay parameters in living biological cells are also described.

Paper Details

Date Published: 1 December 1991
PDF: 9 pages
Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); doi: 10.1117/12.50461
Show Author Affiliations
Alexander J. MacRobert, Imperial College of Science, Technology and Medicine (United Kingdom)
David Phillips, Imperial College of Science, Technology and Medicine (United Kingdom)


Published in SPIE Proceedings Vol. 1439:
International Conference on Scientific Optical Imaging

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