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Proceedings Paper

Stem-loop oligonucleotide beacons as switches for amplifying-fluorescent-polymer-based biological warfare sensors
Author(s): Kenneth D. Clinkenbeard; Akhilesh Ramachandran; Jerry R. Malayer; Joong Ho Moon; Lawrence F. Hancock
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Paper Abstract

Sensors that are exceptionally sensitive with real-time outputs and minimal consumption of reagents are needed to continuously monitor air and water against bioterrorist incidents. Amplifying fluorescent polymers (AFP) provide exceptionally sensitive real-time reagentless sensor platforms as applied to detection of nitroaromatic explosives. This platform technology has the potential to be adapted to detect biological warfare (BW) agents by covalently attaching the 5’ end of stem-loop molecular beacons to AFP as DNA hybridization signal transduction switches. Molecular beacons with loop sequences specific for sequence signatures of a target BW agent are configured with a quencher on the end of the 3’ arm of the stem-loop. The AFP is quenched in the absence of target DNA, but upon hybridization with target the stem is melted, the duplex loop extended, and the AFP dequenched. This signal transduction is reversible upon removal of the target sequence with the molecular beacon reforming the stem-loop conformation. Proof-of-concept research has demonstrated that molecular beacons for signature sequences of Francisella tularensis result in correct identification of the presence of this agent in samples, but no false positives were seen with Escherichia coli.

Paper Details

Date Published: 22 September 2003
PDF: 8 pages
Proc. SPIE 5071, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Defense and Law Enforcement II, (22 September 2003); doi: 10.1117/12.502466
Show Author Affiliations
Kenneth D. Clinkenbeard, Oklahoma State Univ. (United States)
Akhilesh Ramachandran, Oklahoma State Univ. (United States)
Jerry R. Malayer, Oklahoma State Univ. (United States)
Joong Ho Moon, Nomadics, Inc. (United States)
Lawrence F. Hancock, Nomadics, Inc. (United States)

Published in SPIE Proceedings Vol. 5071:
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Defense and Law Enforcement II
Edward M. Carapezza, Editor(s)

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