Share Email Print
cover

Proceedings Paper

Investigation of the hybridization process in DNA microarrays by fluorescence lifetime imaging
Author(s): Daniela Comelli; Cosimo D'Andrea; Paola Taroni; Gianluca Valentini; Rinaldo Cubeddu; Clarissa Consolandi; Gianluca De Bellis; Luigi Rossi Bernardi
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

cDNA-microarrays for expression profiling rely on the assumption that the fluorescence signal is linearly proportional to the concentration of the markers. Yet, interactions amongst different markers and with the substrate might affect the chromophore quantum yield and lead to an erroneous reading. To indirectly evaluate quantum yield changes and their relevance for DNA-microarray reading, we studied the effect on fluorescence lifetime by using a system for fluorescence lifetime imaging. Quenching of the fluorescence emission were observed in the tight environment represented by a DNA microarray due to concentration and to interactions with the processing chemicals present on the substrate.

Paper Details

Date Published: 8 October 2003
PDF: 7 pages
Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); doi: 10.1117/12.501069
Show Author Affiliations
Daniela Comelli, Politecnico di Milano (Italy)
INFM, IFN-CNR (Italy)
Cosimo D'Andrea, Politecnico di Milano (Italy)
INFM, IFN-CNR (Italy)
Paola Taroni, Politecnico di Milano (Italy)
INFM, IFN-CNR (Italy)
Gianluca Valentini, Politecnico di Milano (Italy)
INFM, IFN-CNR (Italy)
Rinaldo Cubeddu, Politecnico di Milano (Italy)
INFM, IFN-CNR (Italy)
Clarissa Consolandi, Univ. degli Studi di Milano (Italy)
CNR-ITBA (Italy)
Gianluca De Bellis, Univ. degli Studi di Milano (Italy)
CNR-ITBA (Italy)
Luigi Rossi Bernardi, Univ. degli Studi di Milano (Italy)
CNR-ITBA (Italy)


Published in SPIE Proceedings Vol. 5141:
Diagnostic Optical Spectroscopy in Biomedicine II
Georges A. Wagnieres, Editor(s)

© SPIE. Terms of Use
Back to Top