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Proceedings Paper

Study on distinction of particles and bubbles for particle counting in liquids by the use fluorescence
Author(s): Takayuki Sakaguchi; Kensei Ehara
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Paper Abstract

In order to find conditions that no bubbles occur on particle counting in liquid, the use of simultaneous detection of scattered light and fluorescence was examined. A liquid particle counter that had fluorescence sensing devices to detect the fluorescence from sample particles was used. Sample particles were dyed and from 1 to 10 micrometer in diameter. The wavelength of laser light to excite fluorescence was 488nm. The fluorescence was filtered by a dichroic mirror and two band pass color filters and detected at 525nm. Although ordinary particle counters detect only scattered light, our system is capable of detecting coincidence of scattered light and fluorescence. By making use of the fact that bubbles make no coincidence signals and particles make coincidence signals we can distinguish bubbles from particles. To evaluate the reliability of the particle counts obtained with this system, we compared them with the counts obtained with the microscopic method.

Paper Details

Date Published: 30 May 2003
PDF: 9 pages
Proc. SPIE 5144, Optical Measurement Systems for Industrial Inspection III, (30 May 2003); doi: 10.1117/12.501013
Show Author Affiliations
Takayuki Sakaguchi, National Institute of Advanced Industrial Science and Technology (Japan)
Kensei Ehara, National Institute of Advanced Industrial Science and Technology (Japan)

Published in SPIE Proceedings Vol. 5144:
Optical Measurement Systems for Industrial Inspection III
Wolfgang Osten; Malgorzata Kujawinska; Katherine Creath, Editor(s)

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