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Proceedings Paper

Cells and tissue imaging with digital holographic microscopy
Author(s): Christian D. Depeursinge; Etienne Cuche; Tristan Colomb; Pia Dahlgren; Anca M. Marian; Frederic Montfort; Pierre Marquet; Pierre J. Magistretti
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Paper Abstract

The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.

Paper Details

Date Published: 14 October 2003
PDF: 5 pages
Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500899
Show Author Affiliations
Christian D. Depeursinge, Ecole Polytechnique Federale de Lausanne (Switzerland)
Etienne Cuche, Ecole Polytechnique Federale de Lausanne (Switzerland)
Tristan Colomb, Ecole Polytechnique Federale de Lausanne (Switzerland)
Pia Dahlgren, Ecole Polytechnique Federale de Lausanne (Switzerland)
Anca M. Marian, Ecole Polytechnique Federale de Lausanne (Switzerland)
Frederic Montfort, Ecole Polytechnique Federale de Lausanne (Switzerland)
Pierre Marquet, Univ. de Lausanne (Switzerland)
Pierre J. Magistretti, Univ. de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 5143:
Novel Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara, Editor(s)

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