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Proceedings Paper

Combined effect of multiple light scattering and radiative transport on fluorescence emission
Author(s): Sandrina P Barbosa; Aleksander Fedorov; Eduardo N Pereira; Jose M. G. Martinho; Mario N Berberan-Santos
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Paper Abstract

The combined effect of multiple light scattering and radiative transport on fluorescence decays is experimentally studied. The experimental setup consists of a 15 cm cubic cell containing a highly fluorescent dye solution and scatterers (either silica particles or milk). The effect of fluorophore concentration was studied, in the absence and presence of scatterer. The effect of excitation and detection geometry was also studied. It is concluded that light scattering can have a significant effect on the fluorescence decays, namely on the mean decay times, and the results can be explained on the basis of the spatial distributions of the successive generations of excited molecules.

Paper Details

Date Published: 15 October 2003
PDF: 8 pages
Proc. SPIE 5138, Photon Migration and Diffuse-Light Imaging, (15 October 2003); doi: 10.1117/12.500711
Show Author Affiliations
Sandrina P Barbosa, Instituto Superior Tecnico (Portugal)
Aleksander Fedorov, Instituto Superior Tecnico (Portugal)
Eduardo N Pereira, Univ. do Minho (Portugal)
Jose M. G. Martinho, Instituto Superior Tecnico (Portugal)
Mario N Berberan-Santos, Instituto Superior Tecnico (Portugal)


Published in SPIE Proceedings Vol. 5138:
Photon Migration and Diffuse-Light Imaging
David A. Boas, Editor(s)

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