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Proceedings Paper

Autofluorescence spectroscopy of colorectal carcinoma: ex vivo study
Author(s): Ladislav Horak; Alexandr Svec; Dimitrij Lezal; Jiri Zavadil
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Paper Abstract

Diagnosis established by means of fluorescence spectroscopy is currently used in the field of urology and bronchology. Its major advantage is that it allows the diagnosis of epithelial dysplasia or malignant proliferation even if routine diagnostic endoscopy fails to reveal any macroscopic changes. The authors present results of their observations that deal with fluorescence diagnosis of colorectal carcinoma. They examined the wet microscopic mounts of healthy colon mucosa and compared them to that prepared from colon mucosa affected by adenocarcinoma. The diagnosis of adenocarcinoma was verified by using clinical and histology means. Fluorescence spectra of tissue samples, excited by means of 488 and 514.5 nm lines of Ar ion laser and/or by He-Ne laser line 632.8 nm, have been studied. This study demonstrated differences in both the spectral shape and in the signal intensity (at unchanged spectral shape) of photoluminescence spectra emitted from tissue affected by adenocarcinoma as compared to that of healthy colon mucosa. The results encourage us to continue the study aimed at development of the diagnostic system usable in the clinical practice.

Paper Details

Date Published: 8 October 2003
PDF: 8 pages
Proc. SPIE 5141, Diagnostic Optical Spectroscopy in Biomedicine II, (8 October 2003); doi: 10.1117/12.500654
Show Author Affiliations
Ladislav Horak, Charles Univ. (Czech Republic)
Alexandr Svec, Charles Univ. (Czech Republic)
Dimitrij Lezal, Institute of Inorganic Chemistry (Czech Republic)
Jiri Zavadil, Institute of Radio Engineering and Electronics (Czech Republic)

Published in SPIE Proceedings Vol. 5141:
Diagnostic Optical Spectroscopy in Biomedicine II
Georges A. Wagnieres, Editor(s)

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