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Proceedings Paper

Use of acceptor fluorescence for determining FRET lifetimes
Author(s): Michael K Hughes; Simon M. Ameer-Beg; Marion Peter; Tony Ng
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Paper Abstract

A technique is presented to utilize time-resolved fluorescence from acceptors in donor-acceptro Forster resonance energy transfer (FRET) fluorophore pairs to improve the efficiency and accuracy of FRET lifetime determinations. This technique was applied experimentally and the results presented. This technique could be useful in fluorescence-liftime imaging systems which can measure more than one wavelength channel simultaneously.

Paper Details

Date Published: 9 October 2003
PDF: 9 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.500641
Show Author Affiliations
Michael K Hughes, King's College London (United Kingdom)
Simon M. Ameer-Beg, Gray Cancer Institute, Mount Vernon Hospital (United Kingdom)
Marion Peter, King's College London (United Kingdom)
Tony Ng, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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